HuDeCA favors workflows of scRNA-seq and single-nucleus (sn)RNA-seq using 10X Genomics Chromium technology. It will also provide a unique imaging platform, combining fluorescent molecular detection techniques in whole-mount human specimens with subsequent 3D imaging and rendering. Time-lapse imaging will be used to study the dynamics of cells in pre-implantation embryos.
A wide range of ultra-high-throughput genomics data will be generated during the project. 10x Genomics Chromium technology is used to obtain RNA and DNA sequencing data.
Single-cell presentation video – © 10x genomics
Molecular signature of different organs obtained by single-cell transcriptomics will be made visible in situ with 3D cell imaging in intact environment, providing new ways of exploring cell populations during human fetal development.
Example of 3D imagery – © Institut de la vision
